Objective lens for brain research by Carl Zeiss

March 19, 2012
The plan-apochromatic 20x/1.0 VIS-IR objective lens from Carl Zeiss Microscopy, LLC acquires 3-D images of nerve cells down to depths of 5.6 mm in intact tissue using a confocal laser scanning or multiphoton microscope, without having to section the brain.

The plan-apochromatic 20x/1.0 VIS-IR objective lens from Carl Zeiss Microscopy, LLC (Thornwood, NY) acquires 3-D images of nerve cells down to depths of 5.6 mm in intact tissue using a confocal laser scanning or multiphoton microscope, without having to section the brain. The lens enables 3-D visualization of the branches of individual nerve cells and the imaging of their connections. A water-based reagent solution transforms the sheath substance of the nerve cells into a transparent matrix without impairing the signals from fluorescent marker and tracer substances.

More Biophotonics Products

-----

PRESS RELEASE

Redesigned Scale Objective Lens from Carl Zeiss for Brain Research

Imaging to depth of 5.6 mm in brain tissue now available

Thornwood N.Y.: The completely redesigned plan-apochromatic 20x/1.0 VIS-IR objective lens from Carl Zeiss now makes it possible to acquire 3D images of nerve cells down to depths of 5.6 mm in intact tissue using a confocal laser scanning or multiphoton microscope, without having to section the brain. This permits the three-dimensional visualization of the branches of individual nerve cells and the imaging of their connections. In untreated tissue, the penetration depth now achievable is five to ten times more that of a multiphoton microscope featuring traditional optics.

Using the microscope to visualize and track nerve cells deep into the brain is an important step in the decoding of the brain circuitry. The new objective lens from Carl Zeiss now brings this goal considerably closer to realization. Carl Zeiss has designed the plan-apochromatic 20x/1.0 VIS-IR objective lens for the clearing method known as Scale developed by Dr. Atsushi Miyawaki at the RIKEN Brain Science Institute in Japan. A special water-based reagent solution transforms the sheath substance of the nerve cells into a transparent matrix without impairing the signals from fluorescent marker and tracer substances.

“I am very impressed by the new possibilities,” says Dr. Atsushi Miyawaki, whose results have been published in Nature Neuroscience and provoked broad interest in the neurosciences. In one of the most important research projects in the field of brain research, the Connectome project, the objective lens will help scientists obtain a better understanding of brain connectivity.

A prototype of the objective lens was presented at Neuroscience 2011 in Washington D.C. After concluding system tests, it will then become available for ZEISS confocal and multiphoton microscopes in 2012.

About Carl Zeiss
Carl Zeiss Microscopy, LLC, offers microscopy solutions and systems for research, routine, and industrial applications. In addition, Carl Zeiss Microscopy markets microscopy systems for the clinical market, as well as optical sensor systems for industrial and pharmaceutical applications. Since 1846, Carl Zeiss has remained committed to enabling science and technology to go beyond what man can see. Today, Carl Zeiss is a global leader in the optical and opto-electronic industries.

With 12,872 current employees and offices in over 30 countries, Carl Zeiss is represented in more than 100 countries with production centers in Europe, North America, Central America and Asia. For more information on the breadth of solutions offered by Carl Zeiss Microscopy, please visit www.zeiss.com/micro.

-----

Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Follow OptoIQ on your iPhone; download the free app here.

Subscribe now to BioOptics World magazine; it's free!

Sponsored Recommendations

Request a quote: Micro 3D Printed Part or microArch micro-precision 3D printers

April 11, 2024
See the results for yourself! We'll print a benchmark part so that you can assess our quality. Just send us your file and we'll get to work.

Request a free Micro 3D Printed sample part

April 11, 2024
The best way to understand the part quality we can achieve is by seeing it first-hand. Request a free 3D printed high-precision sample part.

How to Tune Servo Systems: The Basics

April 10, 2024
Learn how to tune a servo system using frequency-based tools to meet system specifications by watching our webinar!

Precision Motion Control for Sample Manipulation in Ultra-High Resolution Tomography

April 10, 2024
Learn the critical items that designers and engineers must consider when attempting to achieve reliable ultra-high resolution tomography results here!

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!