See the Heart of Your Mouse Beating
BH FLIM systems are capable of recording FLIM at frame rates of 20 fps or more. Data can be recorded by bh’s Express FLIM Technology or, alternatively, by recording a continuous stream of single-photon data from a sample scanned by a fast scanner and extracting subsequent frames from this data stream. The video sequence below demonstrates FLIM at a beating mouse organoid heart.
The data were recorded with a standard bh TCSPC FLIM system integrated in a Zeiss LSM 980 laser scanning microscope. Data acquisition and experiment control was performed by a combination of bh FLIM software with Zeiss ZEN software, transformation into an MP4 video sequence by bh SPCDynamcis.