Adaptable seven-laser flow cytometry system for advanced research applications

The BD Influx Cell Sorting System from BD Biosciences, a segment of BD (Becton, Dickinson and Company; San Jose, CA), allows configuration with up to seven lasers to support two-, four- and six-way sorting to meet specific application or environmental requirements. The system’s modular architecture, exchangeable detector options, hands-on controls and sorting options make it adaptable to a wide variety of site and application needs. The seven laser paths and seven-pinhole optical collection system support 24 parameters simultaneously with a 24 × 24 compensation matrix. Parallel electronics reach a throughput rate of 200,000 events per second, independent of the number of lasers or parameters. Its fluidics design features a special acoustical coupling in the nozzle assembly to create droplets for sorting while ensuring low shear stress to optimize cell viability, even at high pressures. BD FACS Accudrop technology simplifies setup and eliminates manual calculations normally required for drop-delay determination. To support aseptic sorting, the BD Influx System’s disposable fluidics allow researchers to replace a sample line or the complete fluidics path from sheath tank to nozzle tip.