Laser-based instrument measures macromolecular electrophoretic mobilities, challenging protein samples

The Möbius mobility instrument from Wyatt Technology (Santa Barbara, CA) realizes fast measurements of macromolecular electrophoretic mobilities. This laser-based instrument also achieves reproducible measurements of traditionally challenging protein samples, including antibody formulations, bovine serum albumin and lysozyme.

The instrument achieves shortened measurement time and the availability of sufficient data to average away molecular diffusion through massive parallelism of detection and extending the measurable molecular size range below 2 nm. A reduced measurement time of <60 sec, in most cases, helps preserve precious and fragile protein samples.

Simultaneous measurement of the macromolecular hydrodynamic radius is available with the WyattQELS option, which utilizes backward scattered light to determine the sample translational diffusion coefficient. Both reusable flow-through cells and disposable cells are offered for mobility and QELS measurements. Stop-flow is required during mobility measurements. Samples can be introduced by manual injection, an auto-sampler, syringe pump or an auto-titrator. The instrument also features temperature control and can perform automated temperature studies.