Multiphoton microscopy method improves bone metastasis monitoring

Aug. 1, 2018
The system has the goal of gaining a better understanding of and developing treatment for bone metastasis in humans.

Scientists at The University of Texas MD Anderson Cancer Center (Houston, TX) and international colleagues have developed a multiphoton microscopy system that monitors and images cancer that has spread to the bone in mice, with the goal of gaining a better understanding of and developing treatment for bone metastasis in humans.

Related: Clearing of intact bone promises insight into hard tissue

"Advanced prostate cancer and other cancers metastasize to the bones, causing resistance to therapy and pain for patients, but it's not really clear what makes the bone so special to prostate cancer progression," says Eleonora Dondossola, Ph.D., an instructor in Genitourinary Medical Oncology and lead author of the paper that describes the work. "Bone probably provides cues and an attractive microenvironment for cancer cells to grow," she notes, but noninvasive microscopy to study the process in detail is hindered by the thickness of the outer bone blocking the view of inner cavity and bone marrow.

In the paper, the researchers show how the technique can monitor and capture the dynamics of tumor cell interaction with bone and bone resident cells as they occur over time. First, a tissue-engineered construct under a mouse's skin develops in about a month into bone with an internal cavity and a thin outer layer that the microscope can "see" through. Then, after bone marrow and other cells populate the cavity, a cancer cell line is injected. And finally, interactions between malignant cells and bone cells are viewed through the multiphoton microscope via a small glass window sewn into the skin above the bone.

The multiphoton microscopes in the lab of Peter Friedl, MD, Ph.D., professor of Genitourinary Medical Oncology and senior author of the study, can capture up to seven parameters at a time. The bone construct was developed by a team of scientists at the Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, at Queensland University of Technology (Brisbane, Australia), led by Dietmar Hutmacher, Ph.D.

Normal bone biology involves a balance between bone-creating cells called osteoblasts, and bone-destroying cells called osteoclasts, Dondossola notes. Cancer tips this balance, altering the equilibrium between these two populations and leading to symptomatic bone remodeling.

The team's multiphoton microscopy method showed bone loss concentrated around osteoclasts near the tumor. This phenomenon is a known and painful issue for patients with prostate cancer bone metastasis, and a class of drugs called bisphosphonates is used to relieve this symptom. In the clinic, the effect is known to be palliative, relieving pain but not prolonging survival. They treated the mice with the bisphosphonate zoledronic acid and found that the drug did not reduce the number of osteoclasts, but slowed their activity, preserving bone. Notably, the treatment had no effect on tumor growth, and this explains why the bone is stabilized but patient survival is not prolonged.

Friedl's lab is using this model to study cancer treatments in mice, including co-clinical work in immunotherapy and radiation. Drugs that free the immune system to attack cancer are often thwarted by resistance factors in the tumor microenvironment, which the team hopes to observe and characterize.

Full details of the work appear in the journal Science Translational Medicine.

About the Author

BioOptics World Editors

We edited the content of this article, which was contributed by outside sources, to fit our style and substance requirements. (Editor’s Note: BioOptics World has folded as a brand and is now part of Laser Focus World, effective in 2022.)

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