BioMimic optical phantoms from INO (Sainte-Foy, Quebec, Canada) reproduce the characteristics of light propagation (400–900 nm) in living tissue. Based on optical-quality polymers, the optical phantoms can simulate a wide variety of tissue types, and include phantom varieties with VIS-NIR absorbing dyes and TiO2 scatterers. Phantom preparation with a number of layers of differing properties is possible; for fluorescence applications, it is possible to embed fluorescent inclusions with different properties at precise positions in the phantom volume.