The Mirrorball laser-scanning microplate cytometer from TTP LabTech (Herts, UK) can detect low abundance antigens such as cell surface proteins due to high sensitivity. Mix-and-read assays perform hybridoma screening and cell-surface receptor expression. Fluorescence measurements improve object recognition and fewer false negatives based on individual object count. Proprietary low loss optics and red 640 nm laser excitation result in low autofluorescence for high signal-to-noise ratio and data generation.