Semrock's (Rochester, NY) Penta-Sedat multiband fluorescence filter set enables imaging of five distinct fluorophores simultaneously for high-speed imaging into the near-infrared for in-vivo and tissue applications with up to four visible labels. When used in higher-performance microscopes equipped with filter wheels, the quicker analysis time can bring down the overall cost of research and reduce diagnosis time. At the heart of this 11-filter set is an innovative penta-beamsplitter with guaranteed transmission, reflection and edge steepness as effective as single-edge dichroic beamsplitters.