Semrock has introduced a unique line of high-performance fluorescence filter sets optimized for microscopes and imaging systems that use multiple laser excitation. A dual-band filter set for simultaneous 488 and 561 nm laser excitation is ideal for the popular GFP and mCherry fluorescent protein combination. The 488/561 dual-band set provides superb brightness with excellent laser blocking for high contrast when used with laser-based microscopes by researchers doing live-cell, fluorescent-protein-based imaging. A quad-band filter set for 405, 488, 561, and 635 nm laser excitation will be available in April.
Semrock
Rochester, NY
www.semrock.com
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PRESS RELEASE
Semrock Launches Multi-laser Fluorescence Filter Sets
Semrock, the leader in optical filters for the biotech & analytical instrumentation markets, has introduced a unique line of high-performance fluorescence filter sets optimized for microscopes and imaging systems that use multiple laser excitation. A state-of-the-art dual-band filter set for simultaneous 488 and 561 nm laser excitation is ideal for the popular GFP and mCherry fluorescent protein combination. And a remarkable new quad-band filter set for 405, 488, 561, and 635 nm laser excitation ships in April.
Researchers with laser-based microscopes doing live-cell, fluorescent-protein based imaging – in which high sensitivity and high speeds are critical – will find the 488/561 dual-band set provides superb brightness with excellent laser blocking for high contrast. This set is ideal for applications involving GFP and mRFP (especially mCherry) and similar fluorophore combinations, and takes full advantage of newer high-end laser microscopes, or can be used to cost-effectively upgrade the performance of older systems. The 488/561 set is the only hard-coated dual-laser filter set on the market today that offers steep transitions from laser blocking to fluorescence transmission, exceptionally high transmission (> 93%), and deep blocking (OD > 6) at the laser wavelengths to minimize the noise background resulting from residual excitation light. To round out the set, the matched beamsplitter has superior reflection, rapid transitions and high-transmission, low-ripple fluorescence passbands, along with the flatness, low autofluorescence, and surface cosmetic properties desired for superior imaging performance.
The new quad-band laser fluorescence set is designed for simultaneous 405/488/561/635 excitation wavelengths, and is optimal for DAPI/GFP/mCherry/Cy5 and similar fluorophore combinations.
These multi-laser sets are ideal for confocal microscopes such as the Olympus FluoView FV300, FV1000 and the Nikon Eclipse CA Plus/si, AI; for total-internal-reflection-fluorescence (TIRF) systems such as the Nikon TIRF and Olympus FV1000-EVA and TIRF Illuminator; and for spinning-disk confocal microscopes, such as the Olympus DSU system.