Light-tugged molecules reveal molecular forces

Sept. 1, 2008
To better understand how interactions between molecules within a cell regulate such activities as the properties of the cytoskeleton as well as critical division and growth processes, researchers at the Massachusetts Institute of Technology (Cambridge, MA), Stony Brook University (Stony Brook, NY), the University of Kaiserslautern (Kaiserslautern, Germany), and Harvard Medical School (Boston, NY) are tugging at molecules with a spring made of light using optical tweezers.

To better understand how interactions between molecules within a cell regulate such activities as the properties of the cytoskeleton as well as critical division and growth processes, researchers at the Massachusetts Institute of Technology (Cambridge, MA), Stony Brook University (Stony Brook, NY), the University of Kaiserslautern (Kaiserslautern, Germany), and Harvard Medical School (Boston, NY) are tugging at molecules with a spring made of light using optical tweezers.

Actin filaments, a collection of proteins that provide the structural integrity of a cell, are linked together and directed by actin-binding proteins (ABP). To understand the ABP molecular forces, one actin filament was immobilized on a cover-slip surface, and another was tethered to a polystyrene bead that serves as a means to supply a force load from an optical trap. As ABP is introduced to this flow-cell environment, forces between ABP and the two actin filaments (as the ABP unfolds and bonds rupture) can be measured by adjusting the tugging forces of the optical trap and monitoring how far the bead moves out of the optical spring before snapping back to an equilibrium position. Analysis of the measured forces revealed that both ABP unbinding and unfolding are important in regulating cytoskeleton properties with a higher probability of unbinding seen in single ABP-actin filament interactions. Contact Matthew J. Lang at [email protected].

Sponsored Recommendations

Flexible, Thixotropic, One Component Dual Cure Epoxy

Dec. 1, 2023
Master Bond UV23FLDC-80TK is a moderate viscosity, cationic type system that offers both UV light and heat curing mechanisms. It cures readily within 20-30 seconds when exposed...

MRF Polishing

Dec. 1, 2023
Welcome to Avantier, your esteemed partner in optical solutions for over five decades. With a legacy of expert knowledge, we invite you to delve into the realm of precision optics...

Fluorescence Microscopy Part 1: Illuminating Samples for High-Resolution Imaging

Dec. 1, 2023
Illuminating Samples Fluorescence microscopy is a powerful imaging technique widely used in various fields, especially in biomedical research, to visualize and study fluorescently...

Photonics Business Moves: December 1, 2023

Dec. 1, 2023
Here are the top four photonics business moves that made headlines during the week ending December 1, 2023.

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!