open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Optical Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > All Catalog Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Astronomy Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > LRGB Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Narrowband Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Photometry Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Bandpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Longpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Shortpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); 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_t = setTimeout(() => open = false, 75)" > Dichroic Beamsplitters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Emission Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Excitation Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > PCR Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Raman Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Specialty Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Neutral Density (ND) Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Notch Rejection Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Polarizers open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Reflective Mirrors open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > By Wavelength Range open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > UV Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Visible Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > NIR Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > SWIR Filters open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Filter Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > All Catalog Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Astronomy Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > FISH Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Fluorescence Sets open = true, 30)" @mouseleave="clearTimeout(_t); 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_t = setTimeout(() => open = false, 75); clearTimeout(_navT); _navT = setTimeout(() => navOpen = false, 100)"> About Us open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Company open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Mission and Values open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Certifications and Awards open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > History open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > DEIB open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Global Partner Network open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Scientific Communities open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Careers open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Events open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Media open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Image Gallery open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Video Highlights open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Chroma Sponsored Image Contests open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > News open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Testimonials open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)"> Contact Close Total Internal Reflection Fluorescence (TIRF) microscopy restricts excitation to a thin layer near the sample surface, often less than 100 nanometers, dramatically improving signal-to-noise ratio for surface-proximal imaging. That performance depends on filters and dichroics that can handle excitation energy re-entering the emission path at high angles, something standard fluorescence optics aren't built for. This app note covers how evanescent field excitation works, what through-the-lens TIRF demands of optical filters, and how Chroma's substrates, coatings, and cube hardware are built to meet those demands. Get The App Note Read it straight through for the full picture of how TIRF optics work, or keep it as a reference when specifying filters for a TIRF setup. Download the PDF to save, print, or share with your team. How evanescent wave excitation restricts fluorescence to the sample surface The shift from prism-based TIRF to through-the-lens systems Why through-the-lens TIRF places extreme blocking demands on emission filters, exceeding OD 8 Reflected wavefront distortion requirements for TIRF dichroics, and why they're tighter than confocal How Chroma's substrates, coatings, and TIRF-optimized filter cubes are built to meet those requirements Representative TIRF filter set configurations and their spectral performance Who This Is For Plot the transmission curves for representative TIRF filter sets against your fluorochrome and laser lines. 10 Imtec Lane Tel 800-824-7662 Fax 802-428-2525 Close Loading.. Please check the spelling or try searching with different keywords. You can also explore the following sections for more information: Tel: +1-802-428-2500 Toll-free: 800-824-7662 We're here to help you find the optical filter solutions you need. 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