A Practical Guide to Fluorescence and Optical Filters

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open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Optical Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > All Catalog Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Astronomy Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > LRGB Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Narrowband Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Photometry Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Bandpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Longpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Shortpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Single Bandpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Multi-bandpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Edge & Dichroic Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Longpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Shortpass Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Beamsplitters (50/50, etc.) open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Dichroic Beamsplitters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Multi-band Dichroic Beamsplitters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Fluorescence Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Dichroic Beamsplitters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Emission Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Excitation Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > PCR Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Raman Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Specialty Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Neutral Density (ND) Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Notch Rejection Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Polarizers open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Reflective Mirrors open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > By Wavelength Range open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > UV Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Visible Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > NIR Filters open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > SWIR Filters open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Filter Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > All Catalog Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Astronomy Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > FISH Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Fluorescence Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > FRET Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Machine Vision Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Microscopy Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > PCR Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Raman Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Ratiometric Sets open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > TIRF Sets open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Filter Accessories open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Cubes, Sliders and Rings open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Diagnostic Slides open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Reclaimed Cubes open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Catalogs & Downloads open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Custom & OEM Filters open = true, 30); clearTimeout(_navT); navOpen = true" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75); clearTimeout(_navT); _navT = setTimeout(() => navOpen = false, 100)"> Applications open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Aerospace & Defense open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Remote Sensing open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Satellite Communication (SatCom) open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Analytical Spectroscopy open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Raman open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Astronomy & Astrophotography open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Industrial open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Colorimetry open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Machine Vision open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Semiconductor Inspection and Metrology open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Short-Wave Infrared (SWIR) open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > UV open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Life Science & Diagnostics open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Polymerase Chain Reaction (PCR) open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Microscopy & Imaging open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Case Studies open = true, 30); clearTimeout(_navT); navOpen = true" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75); clearTimeout(_navT); _navT = setTimeout(() => navOpen = false, 100)"> Capabilities open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Custom Design & Engineering open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Manufacturing & Optical Coatings open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Metrology & Quality Assurance open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Prototyping & Rapid Development open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > OEM Partnerships open = true, 30); clearTimeout(_navT); navOpen = true" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75); clearTimeout(_navT); _navT = setTimeout(() => navOpen = false, 100)"> Resources open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Customer Support open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Ordering open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Quality & Compliance open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Technical Library open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Tools & Calculators open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > FAQs open = true, 30); clearTimeout(_navT); navOpen = true" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75); clearTimeout(_navT); _navT = setTimeout(() => navOpen = false, 100)"> About Us open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Company open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Mission and Values open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Certifications and Awards open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > History open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > DEIB open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Global Partner Network open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Scientific Communities open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Careers open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Events open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Media open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Image Gallery open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Video Highlights open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)" > Chroma Sponsored Image Contests open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > News open = true, 100)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 200)" > Testimonials open = true, 30)" @mouseleave="clearTimeout(_t); _t = setTimeout(() => open = false, 75)"> Contact Close About Fluorescence: A Practical Guide to Fluorescence and Optical Filters Fluorescence has become one of the most powerful tools in modern optical imaging, and much of what makes it work comes down to precise spectral control. Excitation filters, dichroic beamsplitters, and emission filters work together to isolate a fluorescence signal that can be a million times weaker than the light used to create it. This guide walks through how that system works, from the underlying physics of fluorescence to the filter performance and application-specific considerations that affect real-world results. Get Your Copy Read it straight through for a full grounding in fluorescence filter fundamentals, or keep it on hand as a reference the next time you're selecting a filter set or troubleshooting a signal-to-noise issue. Download the PDF to save, print, or share with your team. What's Inside This guide covers the full path from the physics of fluorescence to the practical filter decisions that affect image quality: How fluorescence works: excitation, emission, and the Stokes shift How excitation filters, dichroics, and emission filters function together in a filter cube What determines filter performance: bandwidth, edge steepness, transmission, and blocking Physical and optical properties that affect real-world results: surface flatness, wedge, angle of incidence, and polarization How filter requirements shift across applications, including FRET, confocal, TIRF, multiphoton microscopy, Raman spectroscopy, quantum dots, flow cytometry, and plate readers Who This Is For Written for researchers, imaging core staff, and instrument designers working with fluorescence, whether you are selecting your first filter set or troubleshooting signal-to-noise issues in an established system. 10 Imtec Lane Tel 800-824-7662 Fax 802-428-2525 Close Loading.. Please check the spelling or try searching with different keywords. You can also explore the following sections for more information: Tel: +1-802-428-2500 Toll-free: 800-824-7662 We're here to help you find the optical filter solutions you need. Filter Sets Individual Filters Filter Accessories Pages & Documents Glossary Terms By using this website, you agree to our use of cookies. We use cookies to improve the user experience and to help our website run effectively. To learn more, read our Privacy Policy . I agree