Monochromatic source
The Polychrome II rapidly tunable excitation source consists of a xenon lamp, galvanometric scanner, blazed grating, and mirrors. The light is coupled to epifluorescent condensers using a solid quart¥fiber. The condensers are available for most microscopes. The unit delivers 4 to 8 mW of optical power in an evenly illuminated field at the objective, with a 12- to 15-nm bandwidth. The device can be tuned to wavelengths from 260 to 680 nm in less than 3 ms.
Applied Scientific Instrumentation, Eugene, OR
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