Jim Passalugo

Business Line Director, Semrock

Jim Passalugo is business line director at Semrock, a unit of IDEX Health & Science LLC (Rochester, NY).

(All panels reproduced with permission per Creative Commons open-access licensing terms [14])
FIGURE 1. A point source of light in the focal plane gets imaged by the microscope objective as an airy disc surrounded by a concentric ring pattern resulting from diffraction (a); when projected in three dimensions, these airy patterns (top panels) comprise PSF (lower panels; b).12, 13 The width and depth of these PSFs dictate maximize x-, y-, and z-resolution, respectively, with higher NA lenses generating smaller airy patterns and PSFs, and thus greater resolution (c).
Detectors & Imaging

Optics/Advanced Microscopy: Practical considerations for advanced microscopy

Ensuring that data generated by advanced imaging methods faithfully represent the biology being studied requires an understanding of key considerations.