Jacco van Rheenen was originally trained in a variety of imaging techniques during his PhD with Dr. Kees Jalink at the Netherlands Cancer Institute. He was among the first to optimize imaging and develop software to quantitatively measure FRET on confocal microscopes. During his PhD in the lab of Dr. Jalink and as postdoc in the lab of Dr. Sonnenberg (Netherlands Cancer Institute) he used several microscopy techniques to study lipid signaling in tumor cells. In order to broader his scales, he obtained a KWF fellowship to do a postdoc in the United States in the lab of Dr. John Condeelis. There he extended his imaging experience by imaging mammary tumors intravitally including two-photon microscopy and became an expert in the field of intravital FRET imaging. In 2008 he was appointed as group leader at the Hubrecht Institute, where he utilizes his imaging techniques to visualize processes that are required for the metastasis of mammary tumor cells in living animals. He was awarded an equipment grant in 2008 and 2011 and a VIDI grant in 2009 from the Dutch Organization of Scientific Research, and a research grant from the Dutch Cancer Society.