Objectives for multiphoton microscopy by Olympus

Nov. 17, 2014
Two new microscope objectives have debuted for deep multiphoton microscopy of life science specimens up to 8 mm beneath the surface.

Olympus (Waltham, MA) has introduced two microscope objectives for deep, high-resolution imaging of life science specimens up to 8 mm beneath the surface. The 10x and 25x objectives have utility in multiphoton microscopy that uses clearing techniques such as CLARITY, SCALEVIEW, and SeeDB, along with live-cell imaging and light-sheet microscopy.

CLARITY-processed Thy1-eYFP-expressing mouse brain. The image was acquired with the FV1200 confocal system using a 515 nm laser and a CLARITY-matched 25x lens. (Image courtesy of Dr. Raju Tomer, Deisseroth Lab, Stanford University)

The objectives are compatible with the company's Fluoview FVMPE-RS and FV1200-MPE multiphoton imaging systems, and can be adapted for use with custom-built light-sheet and other single-plane illumination microscopes. A high transmittance range of 400-1600 nm, extending deep into the infrared (IR) spectrum, allows deep imaging with minimal damage to tissue and can be employed to combine conventional multicolor two- and three-photon imaging with label-free methods such as third- and second-harmonic generation. Able to handle immersion in water, clearing reagents, silicone oil, and others, the 10x objective offers refractive index (RI) immersion capability from 1.33 to 1.52, while the 25x offers RI immersion capability of 1.41 to 1.52.

For more information, please visit www.olympusamerica.com.

More Biophotonics Products

Follow us on Twitter, 'like' us on Facebook, connect with us on Google+, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

Sponsored Recommendations

May 1, 2025
The TSL-775 is a very high specification tunable laser with flexibility in operation to ensure suitability for a wide range of applications.
May 1, 2025
Compare specifications of tunable lasers and find the model you need for your project.
March 31, 2025
Advanced optical filter technologies for satellite communications and remote sensing.
March 31, 2025
Enhance your remote sensing capabilities with Chroma's precision-engineered optical filters, designed for applications such as environmental monitoring, geospatial mapping, and...

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!