Super-resolution Fourier Ptychographic Microscopy
Super-resolution Fourier Ptychographic Microscopy (FPM)
FPM addresses the fundamental trade-off between resolution and field of view in conventional microscopy by combining principles of structured illumination, ptychography, and phase retrieval. This technique enables high-resolution, wide-field imaging using low numerical aperture, and low magnification objective lenses.
Super-resolution Fourier Ptychographic Microscopy Principles
FPM utilizes an array of programmable LEDs for illumination from different angles, expanding the frequency domain bandwidth by overlapping pupil functions. Key components include:
- LED array illumination: Provides angularly varying illumination to capture multiple low-resolution images of the sample from different incident angles.
- Low-NA objective lens: Captures wide field-of-view images at low resolution, which are later computationally enhanced.
- Digital camera: Records the series of low-resolution intensity images corresponding to different illumination angles.
- Computational reconstruction algorithms combine the captured low-resolution images in the Fourier domain to synthesize a high-resolution image with both amplitude and phase information.