November 13, 2006, Livermore, CA--Scientists at Lawrence Livermore National Laboratory (LLNL) for the first time have validated the idea of using extremely short and intense X-ray pulses to capture images of objects such as proteins before the X-rays destroy the sample. The team also established a speed record of 25 femtoseconds for flash imaging.
The new method will be applicable to atomic-resolution imaging of complex biomolecules when even more powerful X-ray lasers, currently under construction, are available. The technique will allow scientists to gain insight into the fields of materials science, plasma physics, biology and medicine.
Using the free-electron laser at Deutsches Elektronen-Synchrotron (DESY; Hamburg, Germany), Livermore scientists, as part of an international collaboration led by LLNL's Henry Chapman and Janos Hajdu of Uppsala University, were able to record a single diffraction pattern of a nanostructured object before the laser destroyed the sample. A Livermore-developed computer algorithm was then used to recreate an image of the object based on the recorded diffraction pattern. This "lensless" imaging technique could be applied to atomic-resolution imaging because it is not limited by the need to build a high-resolution lens. The flash images could resolve features 50 nm in size, which is about 10 times smaller than what is achievable with an optical microscope.
Theory predicts that a single diffraction pattern may be recorded from a large macromolecule, a virus or a cell with an ultra-short and extremely bright X-ray pulse before the sample explodes and turns into a plasma. This means that scientists could better understand the structure of macromolecular proteins without crystallizing them and thus allow rapid study of all classes of proteins.
Livermore scientists, along with colleagues at Uppsala University in Sweden, DESY, Technische Universität Berlin, the Center for Biophotonics Science and Technology at UC Davis, Stanford Synchrotron Radiation Laboratory, and private firm Spiller X-ray Optics of Livermore, conducted the first experimental demonstration of this theory.
Computer simulations based on four different models suggest that a near-atomic resolution structure could be achieved by well-thought out choice of pulse length and intensity of X-ray wavelength before the sample is stripped of its electrons and is destroyed. However, up until now, there had been no experimental verification of the technique.
The experimental demonstration of "flash diffractive imaging" uses the soft X-ray FEL located at the FLASH facility at DESY. FLASH generates high-power soft X-ray pulses by the principle of self-amplification of spontaneous emission. The pulses are 10 million times brighter than today's brightest X-ray sources, synchrotrons. In addition, this experiment showed that it only takes a 25-femtosecond pulse duration to capture the image.
There has been a question whether the diffraction pattern recorded under these circumstances could be reconstructed to obtain undamaged sample information.
"These results could become a standardized method," Chapman said. "This imaging could be applied at the cellular, sub-cellular and down on to single molecule scale."