Super-res pioneer highlights optical tools' relevance for neuroscience

Nov. 27, 2017
Xiaowei Zhuang was surprised by the invitation to present one of four Presidential Special Lectures at Neuroscience 2017.
Barbara G 720

Xiaowei Zhuang was surprised by the invitation to present one of four Presidential Special Lectures at the Society for Neuroscience's 2017 Annual Meeting (Neuroscience 2017; held November 11-15 in Washington, DC). She does not consider herself an expert in neuroscience, she explained—and then she proceeded to captivate the audience with examples of how optical imaging has facilitated discoveries in neurobiology, and promises even more.

Along the way, she paid homage to other super-resolution imaging pioneers, including Sam Hess, developer of fluorescence photoactivation localization microscopy (FPALM), who will present a webcast tomorrow (November 28, 2017) on advances in FPALM and their impact on life sciences. And Zhuang illustrated the important contextual information provided by optical live-cell imaging that other microscopy/nanoscopy methods do not deliver.

Optically aided neuroscience

Zhuang, perhaps best known as co-developer of stochastic optical reconstruction microscopy (STORM),1 is a Harvard University professor and Howard Hughes Medical Institute investigator. Her talk, Illuminating Neurobiology at the Nanoscale and Systems Scale by Imaging, attracted nearly a quarter of the 30,000 attendees to Neuroscience 2017. It presented two techniques: the "old" (circa 2006) super-resolution fluorescence, and a newer (2015) high-throughput single-molecule imaging method for transcriptome analysis in single cells.

She first described how super-resolution approaches—patterned illumination (such as stimulated emission depletion, or STED), and single-molecule (such as STORM)—allow nanometer-scale fluorescence imaging of cells and tissues. Further, she explained how optically enabled illumination of the dynamics of a cell's fine "ultrastructure" provides greater insight than electron microscopy allows.2 Images showing that axon cytoskeletons are made of regularly spaced ring-like structures, not criss-cross patterns (as previously thought), helped make the point.

From super-res to MERFISH

Then, Zhuang introduced the newer method, multiplexed error robust fluorescence in situ hybridization (MERFISH). Developed in her lab, this massively multiplexed single-cell transcriptome imaging approach can profile the expression of thousands of genes—in situ, in a spatially resolved manner—in individual cells (a transcriptome, by the way, represents all messenger RNA molecules expressed in an organism's genes). In an overnight session, for instance, the technique can handle 50,000 cells, and map the spatial organization of the various cell types. This capability should be particularly useful for the BRAIN Initiative's Cell Census project, which NIH had announced just a couple of weeks before.

Zhuang may not be an expert in neuroscience, but she and her collaborators certainly have a lot to offer neuroscientists. And as she made clear, optical methods are critical tools for neuroscientists' toolboxes.


1. M. Rust, M. Bates, and X. Zhuang, Nat. Methods, 3, 10, 793–795 (2006).

2. S. H. Shim et al., Proc. Nat. Acad. Sci., 109, 35, 13978–13983 (2012).

About the Author

Barbara Gefvert | Editor-in-Chief, BioOptics World (2008-2020)

Barbara G. Gefvert has been a science and technology editor and writer since 1987, and served as editor in chief on multiple publications, including Sensors magazine for nearly a decade.

Sponsored Recommendations

Request a quote: Micro 3D Printed Part or microArch micro-precision 3D printers

April 11, 2024
See the results for yourself! We'll print a benchmark part so that you can assess our quality. Just send us your file and we'll get to work.

Request a free Micro 3D Printed sample part

April 11, 2024
The best way to understand the part quality we can achieve is by seeing it first-hand. Request a free 3D printed high-precision sample part.

How to Tune Servo Systems: The Basics

April 10, 2024
Learn how to tune a servo system using frequency-based tools to meet system specifications by watching our webinar!

Precision Motion Control for Sample Manipulation in Ultra-High Resolution Tomography

April 10, 2024
Learn the critical items that designers and engineers must consider when attempting to achieve reliable ultra-high resolution tomography results here!

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!