Frequency-domain FLIM speeds image acquisition

Leutingewolde, The Netherlands--Fluorescence-lifetime imaging microscopy (FLIM) maps the spatial distribution of fluorescence lifetimes--the signature of a fluorescent material--within microscopic images, allowing measurements in living cells as well as in fixed materials. By attaching a homodyne frequency-domain FLIM system to a conventional wide-field fluorescence microscope, lifetime images can be generated within seconds of acquisition time (see Figure).

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