Frequency-domain FLIM speeds image acquisition

April 2, 2008
Leutingewolde, The Netherlands--Fluorescence lifetime imaging microscopy maps the spatial distribution of fluorescence lifetimes within microscopic images, allowing measurements in living cells and fixed materials. By attaching a homodyne frequency-domain FLIM system to a conventional wide-field fluorescence microscope, lifetime images can be generated within seconds. For the complete article, click here.

Leutingewolde, The Netherlands--Fluorescence-lifetime imaging microscopy (FLIM) maps the spatial distribution of fluorescence lifetimes--the signature of a fluorescent material--within microscopic images, allowing measurements in living cells as well as in fixed materials. By attaching a homodyne frequency-domain FLIM system to a conventional wide-field fluorescence microscope, lifetime images can be generated within seconds of acquisition time (see Figure).

For the complete article, click here.

Sponsored Recommendations

March 31, 2025
Enhance your remote sensing capabilities with Chroma's precision-engineered optical filters, designed for applications such as environmental monitoring, geospatial mapping, and...
March 31, 2025
Designed for compatibility with a wide range of systems, Chroma's UV filters are engineered to feature high transmission, superior out-of-band blocking, steep edge transitions...
March 31, 2025
Discover strategies to balance component performance and system design, reducing development time and costs while maximizing efficiency.
March 31, 2025
Explore the essential role of optical filters in enhancing Raman spectroscopy measurements including the various filter types and their applications in improving signal-to-noise...

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!