High-res imaging method quantifies two ligand-binding sites while imaging membrane receptors

An international team of researchers has developed a high-resolution imaging method that allows precise identification and quantification of interactions of a receptor with two ligands simultaneously.

Atomic force microscopy (AFM) is a powerful technique for nanoscale characterization of surfaces. It makes use of a cantilever with an extremely fine tip. Force-distance curve-based atomic force microscopy (FD-based AFM) combines high-resolution imaging and single-molecule force spectroscopy. In studies using biological samples, the AFM tip approaches and retracts from the sample for each pixel. FD-based AFM methods use different coatings of the AFM tip as a toolbox and such methods have made impressive progress in recent years. For the detection of specific binding sites FD-based AFM requires tethering of a ligand to the AFM tip. While contouring protein complexes in a membrane, such functionalized AFM tips can then measure the interactions of the tethered ligand to the protein. It had not been possible to image single-membrane receptors and simultaneously detect their interactions with more than one ligand, but the new method has overcome this hurdle.

For their proof of principle, the team of scientists used the human protease-activated receptor 1 (PAR1), one of the large family of G-protein-coupled membrane receptors (GPCRs). GPCRs mediate most cellular responses to hormones and neurotransmitters, as well as being responsible for vision, olfaction, and taste. GPCRs can coexist in different functional states in the cell membrane and can bind various ligands at different strength or affinity. The GPCR PAR1 is activated by the coagulation protease thrombin, which triggers signaling cascades to initiate cellular responses that help orchestrate hemostasis, thrombosis, inflammation, and possibly also tissue repair. With the aid of their new FD-based AFM method, human PAR1 in proteoliposomes could be imaged while simultaneously detecting extracellular and intracellular interactions of PAR1 with two ligands. The surface chemistry and nanoscopic method developed are applicable to a range of biological systems in vitro and in vivo.

Full details of the work appear in the journal Nature Communications; for more information, please visit http://dx.doi.org/10.1038/ncomms9857.