Kinetic River completes another NIH Phase I SBIR grant

March 25, 2021
Completion of the grant is a milestone towards the automated removal of cellular autofluorescence in flow cytometry.
Unstained and CD45-Alexa 405 stained human eosinophils without removal of autofluorescence (top) and with Colorado autofluorescence removal (bottom). Cells were excited at 375 nm, emission was collected around 460 nm.
Unstained and CD45-Alexa 405 stained human eosinophils without removal of autofluorescence (top) and with Colorado autofluorescence removal (bottom). Cells were excited at 375 nm, emission was collected around 460 nm.

Flow cytometry instrumentation developer Kinetic River (Mountain View, CA) has completed another Small Business Innovation Research (SBIR) project funded by the National Institutes of Health (NIH; Bethesda, MD). The competitive Phase I grant was awarded to the company by the National Institute of General Medical Sciences (NIGMS; also in Bethesda), which fosters research focused on development of innovative biomedical diagnostic and therapeutic platforms.

Flow cytometry is a powerful cell analysis tool with broad utility in research and clinical fields as diverse and important as tumor biology research, cancer and AIDS diagnostics, immunophenotyping, and cancer immunotherapy. Flow cytometry relies heavily on adding fluorescent labels to cells. Accurate measurements of this externally added fluorescence is hindered by the presence of cellular autofluorescence, a nearly ubiquitous endogenous fluorescence inherent to the cells themselves.

The NIH SBIR award funded the development of Kinetic Rivers Colorado automated autofluorescence removal technology. Based on the companys time-resolved flow cytometry technology, the Colorado retains a familiar workflow for the end user, while delivering improved data. By achieving all planned milestones, the company has paved the way for further development and commercialization of this technology.

“Through a combination of hardware and algorithm development, we have been able to demonstrate the removal of the autofluorescence contribution from a population of exogenously stained eosinophils—a cell type with notoriously high autofluorescence signal. The ability to get cleaner signals from such cells will ultimately lead to higher quality data without altering the end-user workflow,” explains Giacomo Vacca, Ph.D., president of Kinetic River.

The successful Phase I program paves the way for further development and commercialization of a 4-laser, 16-channel Colorado instrument with fully automated removal of the contribution from cellular autofluorescence. This is the goal of a Phase II SBIR proposal recently submitted to the NIH.

Source: Kinetic River press release via PR Newswire – March 24, 2021

About the Author

LFW Staff

Published since 1965, Laser Focus World—a brand and magazine for engineers, researchers, scientists, and technical professionals—provides comprehensive global coverage of optoelectronic technologies, applications, and markets. With 80,000+ qualified print subscribers in print and over a half-million annual visitors to our online content, we are the go-to source to access decision makers and stay in-the-know.

Sponsored Recommendations

Request a free Micro 3D Printed sample part

April 11, 2024
The best way to understand the part quality we can achieve is by seeing it first-hand. Request a free 3D printed high-precision sample part.

How to Tune Servo Systems: The Basics

April 10, 2024
Learn how to tune a servo system using frequency-based tools to meet system specifications by watching our webinar!

Motion Scan and Data Collection Methods for Electro-Optic System Testing

April 10, 2024
Learn how different scanning patterns and approaches can be used in measuring an electro-optic sensor performance, by reading our whitepaper here!

How Precision Motion Systems are Shaping the Future of Semiconductor Manufacturing

March 28, 2024
This article highlights the pivotal role precision motion systems play in supporting the latest semiconductor manufacturing trends.

Voice your opinion!

To join the conversation, and become an exclusive member of Laser Focus World, create an account today!