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Wide Field Illumination for Fluorescence Instrumentation

Many fluorescence-based instruments need to analyze or image large areas quickly — from flow cells and multi-well plates, to the entire field of view of a microscope. The throughput of these instruments, however, is often limited by their ability to uniformly illuminate the full field of view with sufficient excitation light of the right wavelengths. As molecular diagnostics and sequencing find increasing application in clinical treatment, throughput is a significant cost barrier to widespread adoption.

One approach to scanning large areas is to illuminate a small area at a time with the desired intensity, stitching together multiple measurements to cover the entire sample area. Alternatively, the same beam can be expanded to cover the full field of view to eliminate scanning, but with a corresponding loss in signal.

The ideal solution, however, is an illumination source that can provide the needed intensity over a sufficiently large field of view to reduce scanning.

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