IMAGE: White-light diffraction tomography (WDT) requires no dyes in the creation of 3D, high-resolution cellular and other object imagery. (Image credit: Gabriel Popescu)
Champaign, IL--A new imaging technique called white-light diffraction tomography (WDT) needs no dyes or other chemicals, yet renders high-resolution, three-dimensional (3D), quantitative imagery of cells and their internal structures--all with conventional microscopes and white light. The team of University of Illinois researchers, led by electrical and computer engineering and bioengineering professor Gabriel Popescu, published their WDT work in Nature Photonics.
"One main focus of imaging cells is trying to understand how they function, or how they respond to treatments, for example, during cancer therapies," Popescu said. "If you need to add dyes or contrast agents to study them, this preparation affects the cells' function itself. It interferes with your study. With our technique, we can see processes as they happen and we don't obstruct their normal behavior."
Because it uses white light, WDT can observe cells in their natural state without exposing them to chemicals, ultraviolet radiation, or mechanical forces--the three main methods used in other microscopy techniques. White light also contains a broad spectrum of wavelengths, thus bypassing the interference issues such as speckle inherent in laser light. The 3D images are a composite of many cross-sectional images, much like an MRI or CT image. The microscope shifts its focus through the depth of the cell, capturing images of various focus planes. Then the computer uses the theoretical model and compiles the images into a coherent 3D rendering.
WDT uses a component that adds onto a conventional phase-contrast microscope, a common piece of equipment in biology labs, without altering the microscope itself. The researchers used conventional microscopes with the intention of making these new optics principles easily accessible for biologists. The researchers hope that this will allow rapid large-scale adoption of WDT, and Popescu founded a startup company, Phi Optics, to help achieve that goal.
The greatest potential of WDT, according to the researchers, is the ability to study cells in 3D over time. Since the cells are not altered, they can be imaged repeatedly, allowing researchers a glimpse into the dynamics of a cell as it goes about its life--or as it is treated with a new drug. A video showcases examples of the 3D images.
"With this imaging we can tell at what scale things within the cell are transported randomly and at what scale processes are actually organized and deterministic," Popescu said. "At first glance, the dynamics looks pretty messy, but then you look at it--we stare at movies for hours and hours--and you realize it all makes sense. Everything is organized perfectly at certain scales. That's what makes a cell alive. Randomness is just nature's way to try new things."
In addition to biological applications, the WDT technique has implications in the broader field of optics as the researchers pushed the boundaries of physics by applying scattering theory to imaging optics.
"We started on this problem two years ago, trying to formulate mathematically the sectioning effect observed in spatial light interference light microscopy (SLIM)," said Renjie Zhou, a graduate student and co-first author of the paper. "We came up with equations which eventually described WDT. The final equation is beautiful and the theory opens opportunities for solving other optics problems in a new theoretical language."
Next, the researchers hope to pursue cross-disciplinary collaborations to explore applications of WDT in biology as well as expansions of the imaging optics demonstrated in WDT. For example, they are using WDT to watch stem cells as they differentiate in hopes of better understanding how they turn into different cell types. Since stem cells are so sensitive, only a chemical-free, non-invasive, white-light technique such as WDT could be used to study them without adverse effects.
SOURCE: University of Illinois; http://news.illinois.edu/news/14/0121WDT_GabrielPopescu.html