Handheld lensless microscope identifies malaria parasites

Los Angeles, CA--In July 2010, Laser Focus World reported that a lensless microscope developed by researchers at the University of California, Los Angeles (UCLA) was being tested in Africa, and we reported again in April 2011 that lensless tomographic 3D imaging using the same technique was demonstrated. Now, the same lensless microscope technique has been used to identify malaria parasites.

The field-portable lensless optical microscope, which can image large sample areas with sub-micron resolution, is based on an imaging technique termed ‘partially-coherent digital in-line holography.’ The method light-emitting diodes (LEDs) and off-the-shelf digital image sensors with no need for lenses or other bulky optical components; the sensor is held in close proximity to the specimen. The process can even be used to image with a cell phone. UCLA uses a digital image processing technique called pixel super-resolution to convert multiple low-resolution microscope images to a single high-resolution one.

During operation, the light reaching the sensor is either scattered from the object or transmitted unaltered through the sample. The sensor records the intensity of the interference pattern between the scattered and undisturbed light, forming a hologram. The phase of the light field is lost because the sensor responds only to intensity, but iterative phase retrieval algorithms enable recovery of the phase. This allows the field to propagate back to the sample plane, which enables recovery of a microscopic image of the object in both the amplitude and phase channels.

The proximity of the sample to the sensor in this configuration allows the use of the entire FOV of the sensor as the microscope's FOV. This area can be orders of magnitude larger than that of a traditional microscope. With a useful magnification 10-40X, the UCLA approach is appropriate for imaging applications that require testing a large sample volume. In fact, the system was used to image red blood cells infected with malaria parasites. Note that a traditional microscope, due to its limited FOV, would require multiple scans to get a statistically significant number of cells to diagnose malaria.

SOURCE: SPIE; http://spie.org/x51571.xml?ArticleID=x51571


Posted by: Gail Overton 

Subscribe now to Laser Focus World magazine; It’s free! 

Follow us on Twitter

Follow OptoIQ on your iPhone. Download the free App here



Most Popular Articles

Webcasts

Laser Measurements Critical to Successful Additive Manufacturing Processes

Maximizing the stability of the variables going into any manufacturing process is what ensures ts consistency and high quality. Specifically, when a laser is...

Handheld Spectrometers

Spectroscopy is a powerful and versatile tool that traditionally often required a large and bulky instrument. The combination of compact optics and modern pa...
White Papers

Miniature Spectrometers for Narrowband Laser Characterization

In less than 60 years, lasers have transformed from the imagined “ray gun” of science fiction int...

Moxtek ICE Cube™ polarizing beamsplitter

The Moxtek ICE CubeTM polarizing beamsplitter (PBS) product performance is compared to a MacNeill...

Simultaneous Intensity Profiling of Multiple Laser Beams Using the BladeCam-XHR Camera

Measurement of multiple laser beams can be a time-consuming process. However, parallel processing...
Technical Digests

REMOTE FIBER-OPTIC SENSING: Data in abundance from difficult environments

The use of optical fibers to measure strain, temperature, and other parameters at desired points ...

SCANNERS FOR MATERIALS PROCESSING: Serving demanding applications

Galvanometer-based scanners are an essential component in laser-based materials-processing system...

OPTICAL COATINGS: Evolving technology produces new benefits

The antireflection, high-reflection, and/or spectral characteristics provided by optical coatings...

FREEFORM OPTICS: Top-notch capabilities lead to expanded possibilities

The use of free-form aspherical surfaces in an optical system can give it abilities impossible to...

Click here to have your products listed in the Laser Focus World Buyers Guide.

PRESS RELEASES

Bristol Instruments Introduces Laser Spectrum Analyzer

09/07/2011 Bristol Instruments Introduces Laser Spectrum Analyzer for Infrared Lasers Complete wavelength an...

New Optical Wavelength Meters

09/07/2011 –Bristol Instruments, Inc., founded by three former employees of Burleigh, has announced the intr...
Social Activity
  •  
  •  
  •  
  •  
  •  
Copyright © 2007-2014. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS