TILL Photonics fluorescence microscope for live tissue imaging

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The dual-color IntraVital2P two-photon imaging fluorescence microscope acquires live tissue images for neurobiology, cell biology, biomedicine and pharmacology. Using the Yanus IV digital scanhead and voice coil-based z focus drive, it scans areas up to 22 mm field diagonally. It has excursions up to 7.5 mm with 50 nm resolution and step response time <10 ms, a 25X objective, and an NA of 1.1.
TILL Photonics
Victor, NY

lisa.riggs@toptica-usa.com

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PRESS RELEASE

TILL Photonics introduces Upright 2-Photon Microscope

Dual-color , 2-Photon Imaging fluorescence microscope, IntraVital2P, acquires the most efficient images of live tissue

TILL Photonics announced today that its new 2-photon fluorescence microscope, IntraVital2P, provides unmatched light collection efficiency for live tissue microscopy. Used mainly in neurobiology, cell biology, biomedicine and pharmacology, the IntraVital2P facilitates imaging at low-light levels and improves SNRs for dynamic measurements deep within highly scattering tissue.

Using TILL’s Yanus IV digital scanhead and voice coil-based z focus drive, the IntraVital2P scans large areas quickly (up to 22mm field diagonal) and acquires exceptional 3D images, allowing long-term, in-vivo imaging with reduced phototoxic effects. This compact, easy-to-use microscope combines excursions of up to 7.5mm with 50nm resolution and a step response time of <10ms, resulting in excellent accuracy. Other performance features include: a 25x objective, NA 1.1, < 1μs pixel dwell time, and digital galvo control.

The Colibri LaserScan open source software provides full control of the setup, the motor and the scanhead, and offers the flexibility of including additional components (e.g., cameras).

Additional benefits of the IntraVital2P include:

  • Unparalleled high-resolution 3D scans
  • Bidirectional scanning with all speeds
  • Non-descanned detection scheme for dual color imaging (with two high-sensitivity GaAsP PMTs of equivalent signal collection efficiency)
  • Unlimited access to conduct simultaneous electrophysiology and imaging.

The Two-Photon Upright microscope will be on display for the first time at the Biophysical Annual Meeting March 6th – 8th in Baltimore at Booth 361.

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Posted by Lee Mather

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